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. 2021 Sep 14;9:720688. doi: 10.3389/fcell.2021.720688

FIGURE 7.

FIGURE 7

Non-canonical WNTs and related component level in SWS-derived cells. (A) qPCR analyses of selective non-canonical Wnt pathway components in SWS-derived fibroblasts. GAPDH was used as an internal control. The graphs represent the 2–ΔΔCt values. Unpaired two-tailed t-test was applied for comparison of each SWS fibroblast with two controls. ****p < 0.0001. Experiments were performed in triplicates with similar results. (B) Western blotting of a few Wnt pathway components. GM05565 and GM09503 are control fibroblasts. P1.1, P4.1, and P5.1 are SWS-derived fibroblasts. (C) Quantitation assay of WNT4 and pJNK band density in (B) and the other replicates. The data are presented as mean ± SEM. An unpaired two-tailed t-test was used. p < 0.05.