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. 2021 Sep 14;8:722113. doi: 10.3389/fvets.2021.722113

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Copyright © 2021 Kaikai, Zhao, Liu, Liu, Huang, Yang, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TRIM25 is induced by duck Tembusu virus (DTMUV) infection or IFN stimulation. qRT-PCR analysis of TRIM25 mRNA expression in duck embryo fibroblasts (A), BHK-21 cells (B), 293T cells (C),and DF-1 cells (D) at 24 h post-infection (hpi) with DTMUV at a multiplicity of infection (MOI) of one. Cells were harvested at 0, 6, 12, and 24 hpi. (E) qRT-PCR analysis of TRIM25 mRNA expression in BHK-21 cells upon stimulation with IFN-α or IFN-β. The expression levels were normalized to GAPDH or β-actin and expressed as fold-change. Data are means ± SEM of three independent experiments.