Table 2.
Overview of the total reads, read length, on-target hits, and the coverage of the target region in rhesus macaque samples.
| Gene fragments (flowcell 1) | |||
|---|---|---|---|
| #1 | #2 | #3 | |
| Total reads | 252981 | 453649 | 2208000 |
| Total reads > 7.000 bp | 25062 | 54772 | 55490 |
| Percentage reads > 7.000 bp | 9.9 | 12.1 | 2.5 |
| Average read length (bp) | 3098 | 3375 | 1978 |
| Average read length (bp) of reads > 7.000 bp | 11258 | 11875 | 9319 |
| Gene to gene fragments (flowcell 2) | |||
| #1 | #2 | - | |
| Total reads | 48586 | 20956 | – |
| Total reads > 7.000 bp | 5179 | 7607 | – |
| Percentage reads > 7.000 bp (%) | 10.7 | 36.3 | – |
| Average read length (bp) | 2884 | 7110 | – |
| Average read length (bp) of reads > 7.000 bp | 12226 | 14789 | – |
| Total reads (flowcells 1 and 2) | |||
| #1 | #2 | #3 | |
| On-target reads (> 7.000 bp reads) | 1070 | 1005 | 292 |
| Percentage on-target reads | 4.3 | 1.8 | 0.5 |
| Mean coverage target region | 60.8 | 91.8 | 26.8 |
| Enrichment factor | 637 | 404 | 128 |
*For #3, a single flowcell was used for all different crRNA pools, generating fragments containing a KIR gene from start to end, and fragments that span from one KIR gene to another.