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. 2021 Aug 23;28(5):dsab014. doi: 10.1093/dnares/dsab014

Figure 2.

Figure 2

Laser microdissection of apomictic and sporophytic cells and differentially expressed genes (DEGs) profiled by RNA-Seq. (A–E) Schematic diagrams of cell lineages in mono- (A) and poly-embryonic (B) ovules at an earlier stage of NEI cell development. (C–E) Nucellar tissue collected separately from the mono- and poly-embryonic ovules at 3 days before anthesis from the F1 progeny derived from a ‘HB pummelo (mono-embryonic) × Fairchild mandarin (poly-embryonic)’ cross. (f) Schematic of cell lineages in a poly-embryonic ovule at a later stage of NEI cell development (7 days after anthesis). Nucellar embryo initial (NEI) cells (G–I) and nucellar sporophytic (SO) cells (J–L) were captured from Ponkan mandarin ovules. The paraffin sections show ovules before microdissection (C, G and J), after microdissection (D, H and K) and the collected cells (E, I and L). (M) Number of expressed genes and DEGs in specific cell lineages. The earlier and later stages of NEI cell development refer to the stages before and after the appearance of nucellar embryo initial (NEI) cells in the nucellus. Poly/Mono-NC, poly/mono-embryonic nucellar tissue. Scale bars 20 μm.