Table 2.

The repeatability and stability of the CVB1-challenged neonatal mouse model.

Experiment	Number of mice	Onset of symptoms (d)	Onset of death (d)	The time of all mice died (d)	Mortality ratio (%)
1	7	3	4	10	100
2	6	3	4	10	100
3	6	3	4	8	100
4	8	4	5	11	100
5	6	3	4	9	100

The results of five replicate and independent experiments that were challenged by a CVB1 clinical stain (10 TCID₅₀ per mouse) in neonatal BALB/c mice at different times are shown. The clinical symptoms and mortality were monitored and recorded daily, which showed good reproducibility.

CVB1 Coxsackievirus B1; TCID₅₀ median tissue culture infective dose.