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. 2021 Sep 14;12:707644. doi: 10.3389/fgene.2021.707644

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Copyright © 2021 Shi, Ding, Zhao, Li, Kang, Tan and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CPSF6 downregulates the expression of VHL. (A) GO analysis of genes with shortened 3′UTR after knockdown of CPSF6 in AGS cells. (B) CPSF6 induced APA shift of VHL. Left, Integrative Genomics Viewer (IGV) genome browser exhibited the poly(A) site usage of VHL 3′UTR. Right, histogram showed the relative expression of the isoform with distal polyadenylation site (dPAS) relative to the one with proximal PAS (pPAS). (C) The mRNA levels of VHL in CPSF6 knockdown AGS and BGC-823 cells. (D) The protein levels of VHL in CPSF6 knockdown AGS and BGC-823 cells. (E) The luciferase reporter assay for the expression activity of the isoform of VHL 3′UTR in AGS and BGC-823 cells. *0.01 < p < 0.05; **0.001 < p < 0.01; ***p < 0.001).