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. 2021 Sep 14;12:707644. doi: 10.3389/fgene.2021.707644

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Copyright © 2021 Shi, Ding, Zhao, Li, Kang, Tan and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The VHL isoform with short 3′UTR inhibits cell growth and promotes apoptosis in GC cells. (A) Western blotting analysis of VHL expression in AGS and BGC-823 cells stably transfected with distinct VHL isoform with short or long 3′UTR. (B,C) Detection of cell proliferation and viability after stably enforced expression of the short- or long-3′UTR isoform of VHL in AGS and BGC-823 cells. (D) Detection of colony formation after stably enforced expression of the VHL isoform with short or long 3′UTR in AGS and BGC-823 cells. (E) Apoptosis ratio was analyzed in AGS and BGC-823 cells. *0.01 < p < 0.05; **0.001 < p < 0.01; ***p < 0.001.