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. 2021 Sep 14;12:723834. doi: 10.3389/fmicb.2021.723834

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Copyright © 2021 Gutiérrez, Rodríguez-Rubio, Ruas-Madiedo, Fernández, Campelo, Briers, Nielsen, Pedersen, Lavigne, García and Rodríguez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibiofilm activity of different engineered proteins against S. aureus 15981 biofilms. (A) Biofilm growth inhibition in the presence of 0.15 μM of the tested proteins. (B) Removal of 8-h S. aureus 15981 biofilms after addition of 1.5 μM of the tested proteins and further incubation at 37°C for 3 h. Bars represent the means ± SDs of six replicates. Bars having distinct lower case letters are statistically different (p < 0.05) from each other according to the ANOVA and SLK post hoc comparison test. The percentage of biofilm removal was calculated by crystal violet staining after the treatment.