Table 2.
Allergen extract standards established by the WHO/IUIS Allergen Standardization Committee.
| Allergen | Status | Availability | Characterization | Reported allergen content | References |
|---|---|---|---|---|---|
| Dog Hair Dander Canis familiaris |
WHO Int. Standard | NIBSC 84/685 | 15 laboratories in 9 countries protein content RAST inhibition CIE/CRIE/RIE IEF SDS-PAGE leukocyte histamine release HCCT RMDT skin testing ()?* |
100 µg Can f 1/ampoule | (21, 22) |
| Short Ragweed pollen Ambrosia artemisiifolia |
WHO Int. Standard | NIBSC 84/581 | 12 laboratories in 5 countries protein content RAST inhibition CIE/CRIE TLIEF leukocyte histamine release HPLC intradermal skin testing (n=5) |
26-40 µg Amb a 1/ampoule (85-133 µg Amb a 1/ml) |
(23) |
| Birch pollen Betula verrucosa |
WHO Int. Standard | NIBSC 84/522 | 20 laboratories in 11 countries protein content RAST inhibition CIE/CRIE/RIE IEF SDS-PAGE leukocyte histamine release skin prick testing (n=20) |
(24) | |
| Timothy grass pollen Phleum pratense |
WHO Int. Standard | NIBSC 82/520 | 14 laboratories in 10 countries protein content RAST inhibition CIE/CRIE/RIE IEF SDS-PAGE HPLC ELISA-inhibition complement inactivation leukocyte histamine release |
(25) | |
| House Dust Mite Der. pteronyssinus |
WHO Int. Standard | NIBSC 82/518 | 19 laboratories in 11 countries RAST inhibition CIE/CRIE/RIE/RIA/SRID IEF intradermal skin testing (n=3) skin prick testing (n=43) leukocyte histamine release ELISA inhibition direct RAST RMDT HCCT |
12.5 µg Der p 1/ampoule 0.4 µg Der p 2/ampoule |
(26–29) |
| Bermuda grass Cynodon dactylon |
International Standard | no longer available |
11 laboratories protein content RAST inhibition CIE/CRIE IEF SDS-PAGE leukocyte histamine release |
(30) | |
| Alternaria Alternaria alternata |
International Standard | no longer available |
30 laboratories protein content RAST inhibition TLIEF HPLC SDS-PAGE/Western Blot CIE/CRIE/RIE/SRID leukocyte histamine release direct RAST skin testing (n=9) |
(31) | |
| Rye grass Lolium perenne |
International Standard | no longer available |
6 laboratories RAST inhibition protein content CIE/CRIE SDS-PAGE/Western Blot IEF skin testing (?)+ ELISA inhibition |
(32) |
*stated on official NIBSC leaflet, but not mentioned in publications.
+statement in (26) that skin testing had been performed, but respective data is not provided.
CIE, crossed immunoelectrophoresis; CRIE, crossed radioimmunoelectrophoresis; HCCT, human complement consumption test; HPLC, high performance liquid chromatography; RIA, radioimmunoassay; RIE, rocket immunoelectrophoresis; RMDT, rat mastocyte degranulation test; SRID, single radial immunodiffusion; TLIEF, thin layer isoelectricfocusing.