Figure 4.

Overexpression of GmWRKY46 enhanced Pi starvation tolerance and lateral roots growth in transgenic Arabidopsis. (A) WT and GmWRKY46-overexpressing transgenic Arabidopsis were grown under three different Pi concentrations (Pi-sufficient, 1 mM Pi; Pi-deficient, 0.2 mM Pi and 62.5 μM Pi) for 15 days on vertically oriented petri plates, respectively. Primary root length (B), lateral root length (C), number of lateral root (D), fresh weight (E), and Pi concentration (F) were determined in WT and transgenic Arabidopsis plants on the 15th day. Values were mean ± SD (n = 3), and asterisks showed that the values were significantly different between the transgenic lines and the WT (Student's t-test, *P < 0.05, **P < 0.01, ***P < 0.001).