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. 2021 Sep 14;12:700651. doi: 10.3389/fpls.2021.700651

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Copyright © 2021 Li, Li, Liu, Ruan, Zheng, Yu, Gai and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of control (CK) and GmWRKY46-overexpressing composite soybean plants (OE) under two Pi level conditions. Composite soybean plants with transgenic hairy roots were transferred to a nutrient solution containing 1 mM Pi (Pi-sufficient) or 2.5 μM Pi (Pi-deficient). After a further 14 days, shoots and roots were separately harvested for analysis. (A) Phenotype of CK and OE. Scale unit: cm. (B) Dry weight of shoots. (C) Dry weight of roots. (D) Total P concentration of shoots. (E) Total P concentration of roots. (F) Root length of composite plants. (G) Number of root tips of composite plants. CK represents the composite plants transformed with the empty vector; OE indicates composite plants with GmWRKY46-overexpressing transgenic hairy roots. DW means dry weight. Data are means ± SD of three biological replicates. Asterisks indicate significant differences between OE and CK (Student's t-test, *P < 0.05; **P < 0.01).