Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 20;22(5):798. doi: 10.3892/ol.2021.13059

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 4. — Long non-coding RNA NUTM2A-AS1 knockdown inhibits NCI-H23 cell viability and induces apoptosis by upregulating miR-590-5p. (A) Viability of NCI-H23 cells was assessed using an MTT assay after 24, 48 and 72 h of incubation. (B) mRNA and (C) protein expression levels of PCNA were assessed using reverse transcription-quantitative PCR and western blotting, respectively. (D and E) Apoptosis was detected using flow cytometry. (F) Cleaved caspase-3 and caspase-3 protein expression levels were measured using western blotting. (G) Cleaved caspase-3/caspase-3 ratio. **P<0.01 vs. control-siRNA; ^##P<0.01 vs. NUTM2A-AS1-siRNA + inhibitor control. NUTM2A-AS1, NUT family member 2A antisense RNA 1; miR, microRNA; siRNA, small interfering RNA; PCNA, proliferating cell nuclear antigen.