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. 2021 Sep 20;22(5):798. doi: 10.3892/ol.2021.13059

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 7. — miR-590-5p inhibits NCI-H23 cell viability and induces apoptosis by downregulating METTL3. (A) Viability of NCI-H23 cells was assessed using an MTT assay after 24, 48 or 72 h of incubation. (B) mRNA and (C) protein expression levels of PCNA were assessed using reverse transcription-quantitative PCR and western blotting, respectively. (D and E) Apoptosis was detected using flow cytometry. (F) Cleaved caspase-3 and caspase-3 protein expression levels were measured using western blotting. (G) Cleaved caspase-3/caspase-3 ratio. **P<0.01 vs. mimic control; ^##P<0.01 vs. miR-590-5p mimic + control-plasmid. miR, microRNA; METTL3, methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit; PCNA, proliferating cell nuclear antigen.