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. 2021 Sep 23;22(5):802. doi: 10.3892/ol.2021.13063

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Overexpression of miR-204-5p inhibits cell mobility and proliferation, and promotes apoptosis. (A) Overexpression of miR-204-5p was confirmed using reverse transcription-quantitative PCR. (B) Effect of overexpressed miR-204-5p on cell proliferation was evaluated using a MTT assay. (C and D) Effect of overexpressed miR-204-5p on apoptosis was assessed using flow cytometry. Statistical results for (E) cell migration and (F) cell invasion. Western blotting results for the protein expression levels of cyclinD1, N-cadherin, vimentin, E-cadherin and Bax in (G) AMC-HN-3 and (H) Tu177 cells. *P<0.05 vs. control group. The data are presented as the mean ± SD of three independent experiments. OD, optical density; miR, microRNA; NC, negative control.