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. 2021 Sep 23;22(5):802. doi: 10.3892/ol.2021.13063

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Inhibitory effects of si-NEAT1 on the progression of laryngeal squamous cell carcinoma are reversed by knockdown of miR-204-5p. (A) Transfection efficiency of inhibitor-miR-204-5p was evaluated using reverse transcription-quantitative PCR. (B) Cell proliferation was measured through MTT assay. Effect of miR-204-5p and NEAT1 on apoptosis was assessed through flow cytometry in (C) AMC-HN-3 and (D) Tu177 cells. (E) Statistical results of the effect of miR-204-5p and NEAT1 on cell migration. (F) Statistical results of the effect of miR-204-5p and NEAT1 on cell invasion. Effect of miR-204-5p and NEAT1 on the protein expression levels of cyclinD1, N-cadherin, vimentin, E-cadherin and Bax in (G) AMC-HN-3 and (H) Tu177 cells was analyzed through western blotting. *P<0.05. The data are presented as the mean ± SD of three independent experiments. OD, optical density; miR, microRNA; NC, negative control; si, small interfering RNA; NEAT1, nuclear enriched abundant transcript 1.