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. 2021 Sep 22;24(5):817. doi: 10.3892/mmr.2021.12457

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Knockdown of CYFIP2 reverses the effect of NUAK2 knockdown on cell migration, invasion and epithelial-to-mesenchymal transition in cervical cancer cells. (A and B) Migration and (A and C) invasion of HeLa cells transfected with shRNA-NUAK2 and shRNA-NUAK2+ si-CYFIP2 were assessed via wound healing and Transwell assays, respectively. (D) Protein expression levels of E-cadherin, N-cadherin, Snail and ZEB1 in HeLa cells transfected with shRNA-NUAK2 and si-CYFIP2 were determined by western blotting. ***P<0.001 vs. Control; ^##P<0.01, ^###P<0.001 vs. shRNA-NUAK2 + si-NC. si, small interfering; shRNA, short hairpin RNA; NC, negative control; NUAK2, NUAK family kinase 2.