Fig. 1.
The dendritic mitochondrial network is stable in the adult living cortex.
A. Fluorescent probes mito-dsRed and GFP targeted in pyramidal neurons of somatosensory cortex using in utero electroporation in mice.
B. Experimental timeline.
C. Imaging of fluorescent probes in fixed tissue section using confocal microscopy (left), in live tissue using transcranial two-photon microscopy (middle) and 3D reconstruction images using Imaris.
D. Time-lapse images of mitochondrial network twice 10 days apart reveals far less remodeling between time points in the dendrite (left) than in the axon (right) (see inset kymographs).
E. Proportion of mitochondria in motion during time lapse imaging of the same dendrites and axons between days, N = 14 segments each, 3 mice.
F. Proportion of mitochondria with coverage remodeling during time lapse imaging of the same dendrites and axons between days, N = 16 and 18 segments respectively, 3 mice. Two-way ANOVA for the dendritic vs axonal coverage of mitochondria F1,32 = 128 post-hoc Sidak test p < 0.0001.