Fig. 3.

Chronic unpredictable stress caused cortical remodeling of the mitochondrial network and behavioral impairment.

A. Experimental timelines.

B. Time of immobility in the tail suspension test (means ± SEM of N = 13 controls, 12 chronic stress, 7 acute retrain stress). One-way ANOVA: F(4,52) = 5.73 p = 0.0007, post-hoc Sidak test *p < 0.05.

C. Time spent in the open arm of the elevated plus maze (means ± SEM of N = 13 controls, 12 chronic stress). Two-way ANOVA: F(1,46) = 5.73 p = 0.0007, post-hoc Tukey test *p < 0.05.

D. Two-photon images of Mito-dsRed in cortex of mice before and after exposure to chronic unpredictable stress (14 days) or acute stress (2-hrs of restrain as the last stressor of the chronic stress protocol).

E. Effect of stress procedures on the proportion of mitochondrial coverage remodeling in dendrites. Student two-tailed T-test comparing day 1 and 14 in controls p = 0.29 and in stress groups ***p < 0.0001 and p = 0.77 respectively, N = 25 dendrites each, 4 mice per group.