FIGURE 5.

Pancreatic β-cells treated with telmisartan exhibit reduced Kv currents and extended APD. (A) Kv currents were recorded in voltage-clamp mode with holding potential from −70 to +80 mV in 10 mV increments. Representative current traces recorded in control and telmisartan-treated (10 μM) β-cells. (B) Current-voltage relationship curves of Kv channels and summary of the mean current density of Kv channels recorded at 80 mV depolarization (control n = 9 cells, telmisartan n = 7 cells). (C) Action potentials were elicited by 4 ms, 150 pA current. Representative action potential waveforms for β-cells treated without or with telmisartan (10 μM) and summary of the mean APDs (n = 7 cells). (D) Representative Kv current traces recorded under treatment of 100 nM Guangxitoxin-1E (GxTX-1E) alone or in combination with 10 μM telmisartan (Tel). (E) Current-voltage relationship curves of Kv channels and summary of the mean current density of Kv channels recorded at 80 mV depolarization (n = 7 cells). The cells compared between the groups in each graph are collected from the same animal. Statistical differences between two groups were determined using an unpaired two-tailed Student’s t test. For comparing the effects of GxTX-1E groups, Tukey Test post hoc analysis was applied. ***p < 0.001, *p < 0.05.