TABLE 2.
Calculation of sensitivities of PCR or reverse transcription-PCR for detection of HSV-1, VZV, and poliovirus type 2 by screen 1
| Virus | Log infectivity (TCID50/ml)a | Log NVP/mlb | Log PCRD50/mlc | NVP/TCID50 | NVP/PCRD50 | TCID50/PCRD50 |
|---|---|---|---|---|---|---|
| HSV-1 | 6.5 | 8.2 | 7.0 | 50 | 16 (158)d | 0.3 (3) |
| Poliovirus type 2 | 7.0 | 8.74 | 8.0 | 55 | 5.5 (34) | 0.1 (3) |
| VZV | 5.0 | 8.3 | 8.0 | 1,995 | 2 (879) | 0.001 (0.1) |
a
TCID50, TCID at which 50% of inoculated monolayers become infected.
b
The number of virus particles (NVP) per milliliter was calculated from electron microscopic counts in parallel with counts of silicon beads by direct proportionality.
c
The reciprocal of the highest dilution positive for nucleic acid that was detectable by PCR adjusted to concentration per milliliter.
d
Data in parentheses refer to the same determinations obtained by multiplex methods previously described by Read et al. (11) and are included for comparison.