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. 2021 Sep 27;19:292. doi: 10.1186/s12951-021-00994-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Characterization of (layered) micelles at different pH-values. A Cryo-TEM and images of HAC-mic, HC-mic and HC-mic layered with PAA (A) or P(NAM-b-AA) (AS) at pH 5. Scale bars in insets represent 100 nm. Micelles in images were analyzed using ImageJ as described in Additional file 1. B + C DLS (B) and ELS (C) measurements of (layered) micelles at equal amine content in acetate-HEPES buffer of pH 5.0 or 7.4. Values represent mean ± SD (n = 2) of z-average (columns), PDI (triangles) or ζ-potential (columns). Bright/gray shades indicate formation of aggregates. D Schematic representation of (layered) micelles at pH 5.0 and 7.4 (left and right side, respectively)