Table I.
Primary antibodies used in western blot analysis.
| Primary antibody | Dilution | Supplier | Cat. no. |
|---|---|---|---|
| BCL6 | 1:500 | ProteinTech Group, Inc. | 21187-1-AP |
| IL-1βa | 1:400 | Affinity Biosciences, Ltd. | AF5103 |
| Caspase-1b | 1:1,000 | Affinity Biosciences, Ltd. | AF5418 |
| ASC | 1:500 | ABclonal Biotech Co., Ltd. | A1170 |
| NLRP3 | 1:1,000 | ABclonal Biotech Co., Ltd. | A5652 |
| β-actin | 1:1,000 | Santa Cruz Biotechnology Co., Ltd. | sc-47778 |
a
IL-1β antibody recognizes both pro-IL-1β (31 kDa) and mature IL-1β (17 kDa)
b
caspase-1 antibody recognizes both pro-caspase-1 (45 kDa) and cleaved caspase-1 (20 kDa). ASC, apoptosisassociated specklike protein containing a CARD; BCL6, B-cell lymphoma 6; NLRP3, nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3.