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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: FASEB J. 2021 Oct;35(10):e21865. doi: 10.1096/fj.202100325RR

Figure 10. GRP94 is acetylated.

Figure 10.

(A) An antibody against acetylated-lysine was used to immunoprecipitate lysates from mIMCD3-WT and mIMCD3-Pkd1KO cells with and without tubastatin-A (T) treatment. Western blots were probed with antibodies to GRP94, HSP90β or HSP90α, α-tubulin and acetylated-α-tubulin. GRP94 and HSP90β are acetylated. HSP90α acetylation was not detected. A marked increase in tubulin acetylation upon treatment with tubastatin-A was appreciated only with antisera directed at the acetylated for of α-tubulin. This experiment was performed three times. (B) An antibody to GRP94 was used for immunoprecipitation of lysates prepared from mIMCD3-WT and mIMCD3-Pkd1KO cells with and without tubastatin-A treatment. The Western blot was probed with an antibody to acetylated lysine. GRP94 is acetylated in both cells. This experiment was performed two times.