Skip to main content
. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: J Neurovirol. 2021 Apr 19;27(3):367–378. doi: 10.1007/s13365-021-00970-4

Fig. 6.

Fig. 6

IDH activity in hNs undergoing nitrosative stress hNs manifest inhibition of isocitrate dehydrogenase (IDH) enzyme activity in their mitochondria due to S-nitrosylation. Wild-type (WT) hiPSC-derived neurons (hNs) were exposed to 100 μM of the physiological NO donor S-nitrosocysteine (SNOC), and the lysates analyzed for IDH activity with isocitrate as substrate in the presence of NADPH with NADP+ being the assayed species reflective of IDH activity. A SpectraMax microplate instrument was used for the measurements. Values are mean + SEM. ∗p < 0.05 by Student’s t test; n = 3.