Table 1.
Peptides with a Large Fold Change in Plasma Stored at 4 °C for 24 h As Compared to Other Preanalytical Conditions
| peptide | precursor charge | UniProt | protein | changea | fold change | p-value | FDR |
|---|---|---|---|---|---|---|---|
| A: Blood at 0 °C for 6 h | |||||||
| SSSYSKQFTSSTSYNRGDSTFESKS | 4 | P02671 | FIBA | + | 2.31 | 0.000 | 0.006 |
| SSSYSKQFTSSTSYNRGDSTFES | 3 | P02671 | FIBA | + | 2.80 | 0.107 | 0.545 |
| GDSTFESKSYKMA | 3 | P02671 | FIBA | + | 1.58 | 0.674 | 1.000 |
| SSSYSKQFTSSTSYNRGDSTFESKSY | 4 | P02671 | FIBA | + | 2.81 | 0.289 | 1.000 |
| B: Blood at RT for 6 h | |||||||
| SSSYSKQFTSSTSYNRGDSTFESKS | 4 | P02671 | FIBA | + | 2.00 | 0.000 | 0.001 |
| SSSYSKQFTSSTSYNRGDSTFES | 3 | P02671 | FIBA | + | 2.02 | 0.989 | 1.000 |
| GDSTFESKSYKMA | 3 | P02671 | FIBA | + | 2.05 | 1.000 | 1.000 |
| SSSYSKQFTSSTSYNRGDSTFESKSY | 4 | P02671 | FIBA | − | 1.08 | 1.000 | 1.000 |
| C: Low centrifugation force | |||||||
| SSSYSKQFTSSTSYNRGDSTFESKS | 4 | P02671 | FIBA | + | 1.08 | 0.047 | 0.433 |
| SSSYSKQFTSSTSYNRGDSTFES | 3 | P02671 | FIBA | − | 1.87 | 0.925 | 1.000 |
| GDSTFESKSYKMA | 3 | P02671 | FIBA | − | 1.03 | 0.961 | 1.000 |
| SSSYSKQFTSSTSYNRGDSTFESKSY | 4 | P02671 | FIBA | − | 1.86 | 0.128 | 0.735 |
| D: Plasma at 4 °C for 24 h | |||||||
| SSSYSKQFTSSTSYNRGDSTFESKS | 4 | P02671 | FIBA | + | 30.69 | 0.000 | 0.000 |
| SSSYSKQFTSSTSYNRGDSTFES | 3 | P02671 | FIBA | + | 38.19 | 0.001 | 0.004 |
| GDSTFESKSYKMA | 3 | P02671 | FIBA | + | 36.85 | 0.023 | 0.082 |
| SSSYSKQFTSSTSYNRGDSTFESKSY | 4 | P02671 | FIBA | + | 84.20 | 0.015 | 0.059 |
| E: Plasma at RT for 24 h | |||||||
| SSSYSKQFTSSTSYNRGDSTFESKS | 4 | P02671 | FIBA | + | 6.20 | 0.000 | 0.000 |
| SSSYSKQFTSSTSYNRGDSTFES | 3 | P02671 | FIBA | + | 2.06 | 0.016 | 0.042 |
| GDSTFESKSYKMA | 3 | P02671 | FIBA | + | 5.42 | 0.030 | 0.069 |
| SSSYSKQFTSSTSYNRGDSTFESKSY | 4 | P02671 | FIBA | + | 3.70 | 0.053 | 0.111 |
a
The symbols + and − indicate an increase or decrease of peptide abundance compared to the control group.