TABLE 1.
Oligonucleotide primers used for detection and isolation of f17A- and f17G-related genes
| Primer | Oligonucleotide sequence (5′ to 3′) | Locationa | Size of PCR product (bp) | Gene detected |
|---|---|---|---|---|
| F17A1 | ATGCAGAAAATTCAATTTATCCT | 1–23 | ||
| F17A2 | ATCATTAATAGCAACTGTATC | 421–441 | 441b | f17A |
| F17A3 | GTTTCATACAGGCTCCGT | 600–617 | 618b | f17A |
| F17A4 | CTATGCAGAAAATTCAATTTATC | −2–21 | 542 | f17A |
| F17A5 | CTGATAAGCGATGGTGTAATT | 520–540 | ||
| F17G1 | AGGTCTTTCTGGCTGTATTCAT | 17–38 | 738 | f17G |
| F17G2 | CTGATAGGAAAACTGAAATGT | 729–753 | ||
| F17G3 | ATGAGGCAATAATATGACAA | −13–7 | 1,302 | f17G |
| F17G4 | TTATCCATGCGCTGACGAAGT | 1278–1298 | ||
| F17G5 | GCTGTTCCGGAAAACCATCATGTA | −20–43 | 1,166 | f17G |
| F17G6 | AACGCTGTTATCCAGCTTCAGAAA | 1100–1123 |
a
Position numbers are from the nucleotide sequence of the f17A cluster from E. coli 25KHO9 (GenBank accession no. AF022140).
b
Size of PCR product combined with primer F17A1.