TABLE 2.
Classification of E. coli strains producing f17-related genes into genotypic subtypes
| Origin and serogroup (no. of strains analyzed) | No. of strains with genotypic subtypea
|
||||
|---|---|---|---|---|---|
| F17dA + F17GI | F17aA + F17GII | F17cA + F17GII | F17dA + F17GII | F17A + F17Gb | |
| Ovine | |||||
| NT (7) | 1 | 0 | 6 | 0 | 0 |
| O6 (1) | 0 | 0 | 1 | 0 | 0 |
| O8 (3) | 0 | 0 | 3 | 0 | 0 |
| O11 (13) | 0 | 0 | 8 | 0 | 5 |
| O23 (2) | 0 | 0 | 2 | 0 | 0 |
| O26 (1) | 0 | 0 | 1 | 0 | 0 |
| 077 (2) | 0 | 0 | 2 | 0 | 0 |
| O101 (2) | 0 | 0 | 1 | 0 | 1 |
| O116 (1) | 0 | 0 | 1 | 0 | 0 |
| O117 (1) | 0 | 0 | 0 | 1 | 0 |
| O138 (1) | 0 | 0 | 0 | 0 | 1 |
| Caprine | |||||
| NT (6) | 0 | 0 | 6 | 0 | 0 |
| O11 (1) | 0 | 0 | 1 | 0 | 0 |
| O44 (3) | 0 | 3 | 0 | 0 | 0 |
| O116 (1) | 0 | 0 | 1 | 0 | 0 |
| Total | 1 | 3 | 33 | 1 | 7 |
a
No. of strains positive by the multiplex PCR (5).
b
No. of strains negative for the multiplex PCR method with a positive amplification by primer pairs F17A1 plus F17A2 (specific for f17A-related genes) and F17G1 plus F17G2 (specific for f17G genes) and with a positive reaction by Southern blot hybridization with probes for F17A and F17G.