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. 2021 Sep 14;8:672270. doi: 10.3389/fvets.2021.672270

Table 3.

Primers used for quantitative real time PCR.

Gene name Accession number Primer sequence 5-3 Product size (bp) Tm (°C)
OTUD1 XM_005500805.2 F: CCATGGGGCAAATGCTGAAC 82 56
R: TGAACCATGGTGGAAACGGT
STARD5 XM_005513899.2 F: GTTCGCTGGCAACTTGTACC 114 56
R: ACGGTTTTGTCCCACTTGGT
CRTAP XM_005502877.2 F: TATTGCGGCAGCTCACACAT 147 57
R: GCCCTGACAAACAGATTCTCG
FAAH XM_005501003.3 F: TGCAGCTGTTGTGCTACTGA 139 56
R: GGTGCCTGGATTCTCTTGCT
DDIT4 XM_021280750.1 F: CTCATCGAGGAGTGCTGACG 116 59
R: ACAGCCCTACTCCAGCCTAA
PGPEP1 XM_021281574.1 F: TTCGTGTGAAAGTGGAGCGA 149 56
R: TGGCTTCAGAACTACTTTCATTTCC
DUSP5 XM_021295890.1 F: GCAGAGTGCCAACCACAAAC 51 58
R: ATTTCAACTGGACCACCCTGG
MSTRG.25503.4 F: CACAAGTCTATGGGGCCAGA 137 56
R: TTTGCCAGCTTCCAGTCAAC
MSTRG.143046.33 F: CGTCTCTTCGCATGCTTAGG 107 58
R: AGCAGAGTCAGTTCCCAGTG
MSTRG.42991.6 F: TGTGGTGTGCAGTTTGATGG 83 55
R: CTGCCAAGGTTTCTGCATT
MSTRG.27973.1 F: ACCAAGATGCTGAAGGGAGT 143 58
R: CTCCATCCTCCTGACACTCC
MSTRG.140096.1 F: CCCTGCCACCCTCTCTTTT 124 59
R: TTTCCAGGTCCCTTCCATCC
MSTRG.144519.21 F: AGTGAGGCCTACATCATCCG 125 58
R: TGATGTGTGTAGGAGGGCTG
MSTRG.95666.15 F: TGCTCCTATTGCCCTTGACA 82 56
R: CCTACCTGCTGTAACCCACT
β-actin XM_005504502.2 F: GAGAAATTGTGCGTGACATCA 152 57
R: CCTGAACCTCTCATTGCCA