FIGURE 3.

Principal coordinate analysis (PCoA) analysis between before (BT) and after transport (AT) groups based on the weighted UniFrac (A) and unweighted UniFrac (B) algorithms. UniFrac PCoA were calculated based on a 97% OTU similarity to show overlap between BT and AT microbiota. Percentage values at the axes indicate contribution of the principal components to the explanation of total variance in the dataset. Weighted UniFrac PCoA (A) shows the first two PCs (PC1 explaining 43.01% of the variance; PC2 explaining 17.02% of the variance). Unweighted UniFrac PCoA (B) shows the first two PCs (PC1 explaining 21.03% of the variance; PC2 explaining 12.26% of the variance). Each sample of BT group (n = 12) is represented by a point in blue and AT group (n = 12) in red. The weighted and unweighted UniFrac distances were compared using analysis of similarities (ANOSIM)