. Author manuscript; available in PMC: 2022 Mar 22.

Published in final edited form as: Nat Metab. 2021 Sep 22;3(9):1217–1227. doi: 10.1038/s42255-021-00449-w

Table 1.

Major morbidities contributing to death in BXD family

Morbidity Type	Diet	n with single morbidity	n with multiple morbidities	p value

Unnatural causes	CD	7	1	0.1257
Unnatural causes	HFD	3	0	0.1257

Non-neoplastic conditions	CD	16	2	0.3715
Non-neoplastic conditions	HFD	22	2	0.3715

Heart/ cardiovascular	CD	0	1	0.0003^**
Heart/ cardiovascular	HFD	8	7	0.0003^**

Polyarteritis (autoimmune)	CD	0	0	0.1204
Polyarteritis (autoimmune)	HFD	3	1	0.1204

Lymphoma	CD	25	3	0.7402
Lymphoma	HFD	26	1	0.7402

Sarcoma	CD	9	2	0.2042
Sarcoma	HFD	4	2	0.2042

Carcinoma	CD	12	3	0.0065^*
Carcinoma	HFD	4	0	0.0065^*

Renal	CD	0	1	0.3673
Renal	HFD	1	3	0.3673

Teratoma	CD	0	1	0.4903
Teratoma	HFD	0	0	0.4903

Significant at nominal alpha of 0.05

^**

Significant with Bonferroni correction for multiple tests

Unnatural causes = flooded cage, foot injury in wire lid, eye abrasion leading to euthanasia, and other.

Miscellaneous non- neoplastic conditions = inflammation, infection, amyloidosis, ulcerative dermatitis

Heart = major contribution of cardiovascular failure other than polyarteritis

Polyarteritis = systemic multifocal arteritis suggestive of autoimmune disease

Lymphoma = hematopoietic neoplasia including lymphoma and histiocytic sarcoma

Sarcoma = nonhistiocytic sarcoma, spindle cell, hemangiosacoma, leiomyosarcoma, sarcoma NOS

Carcinoma = carcinomas (hepatocellular, squamous cell)

Renal = renal failure due to severe nephropathy