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(A) The 32P-cap-labeled actin 309-nt RNA substrate was synthesized either in the presence (+CH3) or absence (-CH3) of the methyl donor S-adenosylmethionine. 0.02 pmol of methylated or unmethylated RNA substrate was incubated with either 50 ng of recombinant wild-type L375 or mutant L375 (E258Q) in mRNA decapping assays as described in Fig 2B.
