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. Author manuscript; available in PMC: 2021 Sep 28.
Published in final edited form as: Sci Transl Med. 2020 Nov 18;12(570):eaay5445. doi: 10.1126/scitranslmed.aay5445

Fig. 1.

Fig. 1.

Detection of cutimycin (ctm), the product of BGC ppa0859–0866, in native and heterologous hosts. A) Arrow representation of the ppa0859–0866 BGC from C. acnes KPA171202. B) HPLC profiles for crude ethyl acetate extracts of select ppa0859–0866 BGC+ and - C. acnes strains. The thiopeptide product of ppa0859–0866, dubbed cutimycin, elutes at 73.5% MeCN as visible in the blue trace from C. acnes HL030PA1. Arrow indicates the cutimycin peak. C) Comparison of HPLC profiles of crude cell extracts of C. glutamicum hosting the cutimycin BGC on a plasmid (blue) versus hosting the empty vector control (red). Arrow indicates the cutimycin peak. D) Structure of the Cutibacterium-produced thiopeptide cutimycin.

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