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. 2021 Jun 30;1(3):100042. doi: 10.1016/j.crmeth.2021.100042

Figure 5.

Figure 5

brp-T2A-QF2w drives expression in the central and peripheral nervous system of Ae. aegypti mosquitoes

(A) Anti-Brp (neuropil) and anti-GFP staining in brain of brp>CD8:GFP female.

(B and C) Anti-Brp and intrinsic Syt1:tdTomato signal in representative z planes from the brain (B) and antennal lobe (C) of brp>Syt1:tdTomato adult female. The extra dots in the red channel likely reflect Syt1:tdTomato fusion proteins retained in the cytosol of some neurons. The high levels of expression achieved by the QF2/QUAS system might exceed the capacity of neurons to translocate all protein to pre-synaptic sites.

(D–F) Intrinsic GFP fluorescence in antenna (D), maxillary palp (E), and labella (F) of brp>CD8:GFP adult females. Labeled cells in the inset of (D) show elongated dendrites characteristic of antennal trichoid sensilla, whereas those in maxillary palp show capped dendrites characteristic of capitate peg sensilla.

(G) Intrinsic GFP fluorescence in brp>CD8:GFP larva showing signal in brain, ventral nerve cord, and segmental ganglia. The larva moved slightly between the consecutive bright-field (left) and fluorescent (right) images. Scale bars, 100 μm (A, B, and D–F) and 50 μm (C).