REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-GFP	ThermoFisher	Cat# A-11122; RRID: AB_221569
Mouse NC82 anti-Brp	DHSB	RRID: AB_2314866
Goat-anti-rabbit Alexa 488	ThermoFisher	Cat# A27034SAMPLE; RRID: AB_2536097
Goat-anti-mouse CF680	Biotium	Cat# 20065-1; RRID: AB_10852820
Goat-anti-mouse Cy3	Jackson ImmunoResearch	Cat# 115-165-062; RRID: AB_2338685
Bacterial and virus strains
Stellar Competent Cells	Clontech	636763
Chemicals, peptides, and recombinant proteins
Octanal	Sigma Aldrich	O5608-100ML
1-Hexanol	Sigma-Aldrich	H13303-100ML
Paraffin oil	Hampton Research	HR3-421
Cas9 protein	PNA Bio	CP01-200
Deposited data
Syt1:GCaMP6s whole brain intrinsic fluorescence	InsectBrainDB	EIN-0000055
Syt1:GCaMP6s antennal lobe intrinsic fluorescence	InsectBrainDB	EIN-0000056
Syt1:GCaMP6s whole brain staining	InsectBrainDB	EIN-0000063
brp>CD8:GFP whole brain staining	InsectBrainDB	EIN-0000052
brp>Syt1:tdTomato whole-brain staining	InsectBrainDB	EIN-0000053
brp>Syt1:tdTomato antennal lobe staining	InsectBrainDB	EIN-0000054
Experimental models: Organisms/strains
Aedes aegypti: Orlando strain	This paper	N/A
Aedes aegypti: Syt1:GCaMP6s	This paper	N/A
Aedes aegypti: brp-T2A-QF2w	This paper	N/A
Aedes aegypti: Syt1-T2A-3XGCaMP6s	This paper	N/A
Aedes aegypti: Syt1-T2A-QF2-QUAS-GCaMP6s	This paper	N/A
Aedes aegypti: Syt1-T2A-GAL4d-UAS-GCaMP6s	This paper	N/A
Aedes aegypti: QUAS-Syt1:tdTomato	This paper	N/A
Oligonucleotides
Target sequence of sgRNAs, see Table S1	This paper	N/A
Primers for evaluating sgRNA efficiency, see Table S1	This paper	N/A
Primers for verifying HDR integration, see Table S3	This paper	N/A
Primers for constructing donor plasmids, see METHOD DETAILS	This paper	N/A
Recombinant DNA
Backbone plasmid: psL1180	Gift from Leslie Vosshall	N/A
pGP-CMV-GCaMP6s	Addgene	#40753
pattB-synaptobrevin-7-QFBDAD-hsp70	Addgene	#46115
pQUAST-mCD8-GFP	Addgene	#24351
pBPGAL4.1Uw	Addgene	#26226
pQF2wWB	Addgene	#61313
Donor plasmid for Syt1:GCaMP6s	This paper, Addgene	#159636
Donor plasmid for brp-T2A-QF2w	This paper, Addgene	#141094
Donor plasmid for Syt1-T2A-3XGCaMP6s	This paper	N/A
Donor plasmid for Syt1-T2A-QF2-QUAS-GCaMP6s	This paper	N/A
Donor plasmid for Syt1-T2A-GAL4d-UAS-GCaMP6s	This paper	N/A
Software and algorithms
CHOPCHOP	Montague et al., 2014	N/A
CRISPR Design	http://crispr.mit.edu	N/A
Fiji ImageJ	https://imagej.net/Fiji	N/A
R	https://www.r-project.org/	N/A
bioconf	Package ‘Hmisc’	N/A
NoRMCorre	Pnevmatikakis and Giovannucci, 2017	N/A
Computational Morphometry Toolkit	http://nitrc.org/projects/cmtk	N/A