REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
TrypLE Select Enzyme (10X), no phenol red	ThermoFisher	A12177-01
Vapor-Lock	Qiagen	981611
NEBuffer 4	New England BioLabs	B7004S
Qiagen Protease	Qiagen	19155
T4 Phage β-glucosyltransferase	New England BioLabs	M0357L
AbaSI	New England BioLabs	R0665S
T4 DNA Ligase	New England BioLabs	M0202M
Adenosine 5’-Triphosphate	New England BioLabs	P0756L
Agencourt AMPure XP	Beckman Coulter	A63880
SuperScript II Reverse Transcriptase	ThermoFisher	18064014
RNaseOUT Recombinant Ribonuclease Inhibitor	ThermoFisher	10777019
NEBNext High-Fidelity 2X PCR Master Mix	New England BioLabs	M0541L
IGEPAL CA-630	Sigma-Aldrich	I8896-50ML
AluI	New England BioLabs	R0137S
BseRI	New England BioLabs	R0581S
Critical commercial assays
MEGAscript T7 Transcription Kit	ThermoFisher	AMB13345
Deposited data
Single-cell 5hmC sequencing data and scH&G-seq data	This paper	GEO: GSE131678
Experimental models: cell lines
Human: H9	WiCell	WA09
Experimental models: organisms/strains
Mouse: C57BL/6J	The Jackson Laboratory	000664
Mouse: CBA/J	The Jackson Laboratory	000656
Oligonucleotides
Double-stranded adapters for scAba-Seq	Mooijman et al., 2016	N/A
RandomhexRT primer	Hashimshony et al., 2016	N/A
Illumina sequencing primers	Hashimshony et al., 2016	N/A
Blunt-end adapter	scDam&T	NA
Software and algorithms
scPECLR (MATLAB)	This paper	N/A
scH&G-seq	This paper	https://github.com/alexchialastri/scH-G-seq