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. Author manuscript; available in PMC: 2022 Aug 23.
Published in final edited form as: Curr Biol. 2021 Jul 8;31(16):3586–3600.e11. doi: 10.1016/j.cub.2021.06.021

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
rat anti-HA Roche Cat#11867423001
goat anti-rat IRDye 680RD Licor Cat#925-68076;
Lot#C61115-06
streptavidin-IRDye 800CW Licor Cat#925-32230;
Lot#C60913-04
mouse anti-HA Abcam Cat#ab130275 [16B12];
Lot#GR250145-5
mouse anti-Myc Abcam Cat#ab32[9E10];
Lot#GR3272830
rabbit anti-GFP Abcam Cat#ab6556;
Lot#6R3271077-1
mouse anti-PAR-3 DSHB Cat#p4a1; RRID: AB528424
rabbit anti-GIP-1 GenScript7 N/A
CY3-anti-mouse Jackson Immunoresearch Laboratories Cat#115-165-166;
Lot#117091
streptavidin Alexa Fluor 488 Invitrogen Ref#532354;
Lot#1719656
DAPI Sigma N/A
647-anti-rabbit Jackson Immunoresearch Laboratories Cat#711-605-152
488-anti-mouse Jackson Immunoresearch Laboratories Cat#115-545-166
488 anti-rabbit Thermofisher scientific Cat#A11034
Cy5 anti-mouse Jackson Immunoresearch Laboratories Cat#715-175-151
Bacterial and virus strains
E. coli (MG1655 bioB::kan) biotin auxotrophic food Dr. John E. Cronan, University of Illinois N/A
E. coli OP50 standard food CGC N/A
E. coli NA22 standard food CGC N/A
Critical commercial assays
Pierce 660nm Protein Assay Kit Thermo Fisher Scientific Cat#22662
Pierce Streptavidin Magnetic Beads Thermo Fisher Scientific Cat#88817
Q5® Site-Directed Mutagenesis kit New England BioLabs Cat#E0554S
NEBuilder® HiFi DNA Assembly New England BioLabs Cat#E5520S
Deposited data
control_df (compressed.csv with microtubule positions and angles in the wild type C. elegans embryos) This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/control_df
VAB_10Bgut_minus_df (compressed.csv with microtubule positions and angles in the VAB-10Bgut(−) C. elegans embryos) This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/VAB_10Bgut_minus_df
WDR_62gut_minus_df (compressed.csv with microtubule positions and angles in the WDR-62gut(−) C. elegans embryos) This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/WDR_62gut_minus_df
Experimental models: Organisms/strains
C. elegans strain N2 wild-type CGC N/A
C. elegans strain JLF696: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; ptrn-1(wow4[ptrn-1::gfp]) X; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF291: wowEx68(myo-2p::mCherry::unc-54 3’UTR; ges-1p::3xHA::TurboID::unc-54 3’UTR) 25 CGC
C. elegans strain JLF337: wowIs24(myo-2p::mCherry::unc-54 3’UTR;ges-1p::ptrn-1a::3xHA::TurboID::unc-54 3’UTR) This study N/A
C. elegans strain JLF371: wowIs26(myo-2p::mCherry::unc-54 3’UTR; ges-1p::ptrn-1a::3xHA::TurboID::unc-54 3’UTR) This study N/A
C. elegans strain JLF372: wowIs17(myo-2p::mCherry::unc-54 3’UTR; ges-1p::3xHA::TurboID::unc-54 3’UTR) This study N/A
C. elegans strain JLF525: vab-10b(wow80[vab-10b::zf::gfp]) I; zif-1(gk117) III This study N/A
C. elegans strain JLF551: zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V This study N/A
C. elegans strain JLF504: zif-1(gk117) III; par-1(wow87[par-1::zf::gfp]) V This study N/A
C. elegans strain JLF690: zif-1(gk117) III; sma-1(wow102[sma-1::zf::gfp]) V This study N/A
C. elegans strain JLF621: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; eps-8(wow101[eps-8a::zf::gfp]) IV This study N/A
C. elegans strain JLF15: ptrn-1(wow4[ptrn-1::gfp]) X This study N/A
C. elegans strain TV26135: zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wyIs900(unc-122p::rfp; lin-32p::mCherry::PLCdeltaPH) This study N/A
C. elegans strain JLF644: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF589: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF666: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF685: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF701: ebp-2(wow47[ebp-2::gfp]) II; par-3(it300[par-3::mCherry]) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study/CGC N/A
C. elegans strain JLF599: vab-10b(wow80[vab-10b::zf::gfp]) I; ebp-2(wow47[ebp-2::gfp]) II; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF718: ebp-2(wow47[ebp-2::gfp]) II; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF667: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; gip-1[wow3(gfp::gip-1)] zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF597: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; gip-1[wow3(gfp::gip-1)] zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF658: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; gip-1[wow3(gfp::gip-1)] zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF699: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; ptrn-1(wow4[ptrn-1::gfp]) X; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF700: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; wdr-62(wow94[wdr-62::zf::gfp]) V; ptrn-1(wow4[ptrn-1::gfp]) X; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF714: vab-10b(wow80[vab-10b::zf::gfp]) I; zif-1(gk117) III; wdr-62(wow111[wdr-62::tag-rfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF439: ItSi910[elt-2p::vhhgfp4::zif-1::operon-linker::mCherry::histone::tbb-2 3’UTR; cb-unc-119(+)] II; zif-1(gk117) III This study/CGC N/A
C. elegans strain JLF473: vab-10b(wow80[vab-10b::zf::gfp]) I; ItSi910[elt-2p::vhhgfp4::zif-1::operon-linker::mCherry::histone::tbb-2 3’UTR; cb-unc-119(+)] II; zif-1(gk117) III This study N/A
C. elegans strain JLF657: tbb-2(tj26[gfp::tbb-2]) unc-119(ed3) par-3(it300[par-3::mCherry]) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF757: wowIs28(myo-2p::mCherry::unc-54 3’UTR ;elt-2p::zif-1::unc-54 3’UTR;end-1p::histone1::mCherry)II; par-3(wow59[par-3::zf::gfp]) zif-1(gk117) III/qC1; wdr-62(wow111[wdr-62::tag-rfp]) V This study N/A
C. elegans strain JLF442: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; opIs310[ced-1p::yfp::act-5::let-853 3’UTR + unc-119(+)] This study/CGC N/A
C. elegans strain SA899: tbb-2(tj26[gfp::tbb-2]) unc-119(ed3) III; tjIs72[pie-1p::cherry::h2b, pie-1p::cherry::tbg-1, unc-119(+)] Asako Sugimoto/CGC N/A
C. elegans strain JLF673: ItSi910[elt-2p::vhhgfp4::zif-1::operon-linker::mCherry::histone::tbb-2 3’UTR; cb-unc-119(+)] II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V This study N/A
C. elegans strain JLF681: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; sma-1(wow102[sma-1::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF616: ItSi910[elt-2p::vhhgfp4::zif-1::operon-linker::mCherry::histone::tbb-2 3’UTR; cb-unc-119(+)] II ;zif-1(gk117) III; sma-1(wow102[sma-1::zf::gfp]) V This study N/A
C. elegans strain JLF694: eps-8(wow101[eps-8a::zf::gfp]) IV This study N/A
C. elegans strain JLF692: ItSi910[elt-2p::vhhgfp4::zif-1::operon-linker::mCherry::histone::tbb-2 3’UTR; cb-unc-119(+)] II; eps-8(wow101[eps-8a::zf::gfp])/tmC9 IV This study N/A
C. elegans strain JLF1024: zif-1(gk117) III; wow112(zf::gfp::wdr-62) V This study N/A
C. elegans strain JLF648: sma-1(wow102[sma-1::zf::gfp]) V; TH110[pie-1p::par-6::mCherry] This study N/A
C. elegans strain JLF1021: vab-10b(wow157[vab-10b::gfp::sqt-1::hygR]) I; wowIs28(myo-2p::mCherry::unc-54 3’UTR; elt-2p::zif-1::unc-54 3’UTR; end-1p::histone1::mCherry) II; par-3(wow59[par-3::zf::gfp]) zif-1(gk117) III/qC1 This study N/A
C. elegans strain JLF1026: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR); opIs310[ced-1p::yfp::act-5::let-853 3’UTR + unc-119(+)] This study N/A
C. elegans strain JLF1027: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR); opIs310[ced-1p::yfp::act-5::let-853 3’UTR + unc-119(+)] This study N/A
C. elegans strain JLF1028: zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR); opIs310[ced-1p::yfp::act-5::let-853 3’UTR + unc-119(+)] This study N/A
C. elegans strain JLF1029: vab-10b(wow80[vab-10b::zf::gfp]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR); opIs310[ced-1p::yfp::act-5::let-853 3’UTR + unc-119(+)] This study N/A
C. elegans strain JLF1030: vab-10b(wow157[vab-10b::gfp::sqt-1::hygR]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
C. elegans strain JLF1031: vab-10b(wow157[vab-10b::gfp::sqt-1::hygR]) I; zuIs278(pie-1p::cherry::tba-1::pie-1 3’UTR) II; zif-1(gk117) III; wdr-62(wow94[wdr-62::zf::gfp]) V; wowEx111(myo-2p::mCherry; end-1p::bfp::caax; elt-2p::zif-1::unc-54 3’UTR) This study N/A
Oligonucleotides
Sequences for CRISPR/Cas9 edits, see Table S4 This paper N/A
Recombinant DNA
Plasmid pAS31 [ges-1p::3xHA::TurboID::unc-54 3’UTR] Addgene Addgene plasmid # 118220; http://n2t.net/addgene:118220; RRID: Addgene_118220
Plasmid pAS33 [ges-1p::ptrn-1a::3xHA::TurboID::unc-54 3’UTR] This study N/A
Plasmid SA109 [elt-2p::zif-1::unc-54 3’UTR] Addgene Addgene plasmid # 59783; http://n2t.net/addgene:59783; RRID: Addgene_59783
Plasmid pMP1 [end-1p::mtag::bfp::caax] This study N/A
Plasmid pCFJ90 [myo-2p::mcherry::unc-54 3’UTR] Addgene Addgene plasmid # 19327; http://n2t.net/addgene:19327; RRID:Addgene_19327
Plasmid pJF248 [end-1p::histone1::mcherry] This study N/A
Plasmid pJL188 [lin-32p::mcherry::PLCΔPH] This study N/A
Plasmid pDD162 [eft-3p::Cas9 + Empty sgRNA] Addgene Addgene plasmid # 47549; http://n2t.net/addgene:47549; RRID: Addgene_47549
Plasmid pJF250 [ZF::GFP::3xFlag Empty repair template] 20 N/A
Plasmid pDD282 [GFP::3xFlag Empty repair template] Addgene Addgene plasmid # 66823; http://n2t.net/addgene:66823; RRID: Addgene_66823
Plasmid pDD286 [TagRFP-T::3xMyc Empty repair template] Addgene Addgene plasmid # 70684; http://n2t.net/addgene:70684; RRID: Addgene_70684
Plasmid pAS45 [eft-3p::Cas9 + vab-10b sgRNA] This paper N/A
Plasmid pAS62 [eft-3p::Cas9 + wdr-62 sgRNA] This paper N/A
Plasmid pAS78 [eft-3p::Cas9 + wdr-62 sgRNA] This paper N/A
Plasmid pMP44 [eft-3p::Cas9 + par-1 sgRNA] This paper N/A
Plasmid pMP15 [eft-3p::Cas9 + par-3 sgRNA] This paper N/A
Plasmid pLC003 [eft-3p::Cas9 + sma-1 sgRNA] This paper N/A
Plasmid pLC007 [eft-3p::Cas9 + eps-8 sgRNA] This paper N/A
Plasmid pAS46 [ZF::GFP::3xFlag with vab-10b repair template] This paper N/A
Plasmid pAS61 [ZF::GFP::3xFlag with wdr-62 repair template] This paper N/A
Plasmid pAS82 [ZF::GFP::3xFlag with wdr-62 repair template] This paper N/A
Plasmid pAS77 [TagRFP-T::3xMyc with wdr-62 repair template] This paper N/A
Plasmid pMP43 [ZF::GFP::3xFlag with par-1 repair template] This paper N/A
Plasmid pMP14 [ZF::GFP::3xFlag with par-3 repair template] This paper N/A
Plasmid pLC002 [ZF::GFP::3xFlag with sma-1 repair template] This paper N/A
Plasmid pLC006 [ZF::GFP::3xFlag with eps-8 repair template] This paper N/A
Plasmid pAS57 [GFP::3xFlag with vab-10b repair template] This paper N/A
Software and algorithms
Image J (version 2.1.0) NIH https://imagej.nih.gov/ij/
Prism 9 for macOS (version 9.1.0) GraphPad https://www.graphpad.com
NIS elements Nikon Instruments https://www.microscope.healthcare.nikon.com/products/software/nis-elements
Illustrator Adobe https://www.adobe.com
InDesign Adobe https://www.adobe.com
Photoshop Adobe https://www.adobe.com
WDSPdb 2.0 66 http://www.wdspdb.com/wdsp/
JalView v2.11.1.0 83 https://www.jalview.org
Clustel Omega v1.2.2 82 https://www.ebi.ac.uk/Tools/msa/clustalo/
Gblocks v0.91 84 http://molevol.cmima.csic.es/castresana/Gblocks.html
PhyML 3.0 85 http://www.atgc-montpellier.fr/phyml/
SMS for PhyML 86 http://www.atgc-montpellier.fr/sms/
FigTree v1.4.4 Andrew Rambaut http://tree.bio.ed.ac.uk/software/figtree/
Worm tissue expression prediction server 30 http://worm.princeton.edu/
GO term finder 71 https://go.princeton.edu/cgi-bin/GOTermFinder
Biomart 73 http://biomart.org
STRING 32 http://string-db.org
Python 3.7, Sanchez_etal_python_environment.yaml
The Python 3.7 environment used to run all the analysis. This.yaml file includes all the package versions and dependencies. 		https://www.anaconda.com https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/Sanchez_etal_python_environment.yaml
Python 3.7, microtubule_tracking_class.py
A class that includes all the microtubule segmentation and tracking functions 		This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/microtubule_tracking_class.py
Python 3.7, run_microtubule_tracking_class_example.py
An example on how to use the ‘microtubule_tracking_class’ 		This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/run_microtubule_tracking_class_example.py
Python 3.7, Sanchez_etal_figure_functions.py
The functions used to plot Figures 4E, S6G-H 		This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/Sanchez_etal_figure_functions.py
Python 3.7, Sanchez_etal_plot_figures.py
The script which runs the ‘Sanchez_etal_figure_functions’ to plot the data 		This paper https://github.com/JacobsWagnerLab/published/blob/master/Sanchez_2020/Sanchez_etal_plot_figures.py