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. 2021 Sep 28;10:e68479. doi: 10.7554/eLife.68479

Figure 2. Read coverage over the SARS-CoV-2 genome using Tiled-ClickSeq.

(A) Read coverage obtained from Tiled-ClickSeq over the whole viral genome is depicted when sequencing using an Illumina MiSeq (orange) or on an Oxford Nanopore Technologies MinION device (blue). A ‘saw-tooth’ pattern of coverage is observed with ‘teeth’ upstream of tiled-primers, indicated at the bottom of the plot by short black lines. (B) Zoomed in read coverage of nts 1–2400 of the SARS-CoV-2 genome with coverage of Illumina MiSeq reads from five individual primers coloured to illustrate coverage from downstream amplicons overlapping the primer-binding sites of upstream tiled-primers (Blue: Read coverage from primer 1; Orange: coverage from primer 2; Green: coverage from primer 3; Red: coverage from primer 4; Purple: coverage from primer 5).

Figure 2.

Figure 2—figure supplement 1. Read coverage of tiled nanopore data over 12 SARS-CoV-2 isolates.

Figure 2—figure supplement 1.

(A) Read coverage obtained from Tiled-ClickSeq over the whole viral genome for 12 World Reference Center for Emerging Viruses and Arboviruses (WRCEVA) isolates is depicted when using an Oxford Nanopore Technologies MinION device. Tiled-primers (v1) are indicated at the bottom of the plot by short blue lines. (B) Read count mapping statistics for each isolate are shown in the table.
Figure 2—figure supplement 2. Read coverage of tiled ARTIC data over 12 SARS-CoV-2 isolates: (A) Read coverage obtained from ARTIC sequencing protocol over the whole viral genome for 12 WRCEVA isolates is depicted when sequenced on an Illumina NextSeq.

Figure 2—figure supplement 2.