| Cell line (Homo sapiens) |
HEK Lenti-X 293T (modified) |
Takara Bio |
632,180 |
Genomically integrated TRE3G-GFP |
| Commercial assay or kit |
iScript cDNA Synthesis Kit |
Bio-Rad |
1708890 |
|
| Commercial assay or kit |
RNeasey Plus Mini Kit |
Qiagen |
74,134 |
|
| Commercial assay or kit |
iTaq Universal SYBR Green Supermix |
Bio-Rad |
1725120 |
|
| Peptide, recombinant protein |
T7 DNA Ligase |
NEB |
M0318S |
|
| Peptide, recombinant protein |
T4 Polynucleotide Kinase |
NEB |
M0201L |
|
| Commercial assay or kit |
NucleoSpin Gel and PCR Clean-up |
Takara |
32–740609.50 |
|
| Commercial assay or kit |
In-Fusion HD Cloning Plus |
Takara |
638,920 |
|
| Commercial assay or kit |
KAPA HiFi Hot-start PCR kit |
Roche |
07958897001 |
|
| Commercial assay or kit |
HiScribe T7 Quick High Yield RNA Synthesis Kit |
NEB |
E2040S |
|
| Commercial assay or kit |
MEGAclear Transcription Clean-Up Kit |
Thermo Fisher |
AM1908 |
|
| Peptide, recombinant protein |
EnGen Lba Cas12a (Cpf1) - 2000 pmol |
NEB |
M0653T |
|
| Peptide, recombinant protein |
Recombinant RNase inhibitor |
Takara Bio |
2313 A |
|
| Commercial assay or kit |
RNA Nano 6,000 chips |
Agilent |
5067–1511 |
|
| Sequence-based reagent |
Low Range ssRNA Ladder |
NEB |
N0364S |
|
| Commercial assay or kit |
TransIT-LT1 transfection reagent |
Mirus Bio |
MIR2304-70 |
|
