TABLE 1.
Reaction specificity of AEP14349
| Componenta | Sp actd (μmol min−1 mg–1) |
|
|---|---|---|
| l-His | l-Gln | |
| 2-OG,b VC,c Fe2+, enzyme | 0.208 ± 0.024 | 0.262 ± 0.023 |
| VC, Fe2+, enzyme | 0 | 0 |
| 2-OG, Fe2+, enzyme | 0.206 ± 0.020 | 0.051 ± 0.003 |
| 2-OG, VC, enzyme | 0.025 ± 0.002 | 0.030 ± 0.002 |
| 2-OG, VC, Fe2+ | 0 | 0 |
| 2-OG, VC, Fe2+, EDTA, enzyme | 0 | 0 |
| NADH, VC, Fe2+, enzyme | 0 | 0 |
| NADPH, VC, Fe2+, enzyme | 0 | 0 |
l-His or l-Gln was included in each reaction.
2-Oxoglutarate.
cl-Ascorbic acid.
Each concentration is described in Materials and Methods. Data are presented as the means ± SD from the results of three independent experiments.