FIG 4.

Mn supplementation rescues the growth defect of the ΔtroR mutant under Zn or Co excess conditions. (A to C) The WT, ΔtroR, and CΔtroR strains were grown in TSBS medium supplemented with 100 μM Zn alone (A), 100 μM Zn with 0.25 mM Mn (B), or 0.5 mM Mn (C). (D to F) The WT, ΔtroR, and CΔtroR strains were grown in TSBS medium supplemented with 1 mM Co alone (D), 1 mM Co with 0.5 mM Mn (E), or 1 mM Mn (F). (G to I) The WT, ΔtroR, and CΔtroR strains were grown in TSBS medium supplemented with 1.5 mM Cu alone (G), 1.5 mM Cu with 1 mM Mn (H), or 2 mM Mn (I). The strains were grown in 96-well plates (200 μl per well) at 37°C with linear shaking, and the OD₅₉₅ values were measured hourly. At least three independent experiments were performed; the data shown are the means ± SDs from three wells in a representative experiment.