FIG 5.

TroR is required for S. suis growth in metal-depleted medium supplemented with excess Mn. The WT, ΔtroR, and CΔtroR strains were grown in Chelex-treated TSBS medium supplemented with deionized water (A), 1 mM Mn (B), 2 mM Mn (C), or 4 mM Mn (D). The strains were grown in 96-well plates (200 μl per well) at 37°C with linear shaking, and the OD₅₉₅ values were measured hourly. At least three independent experiments were performed; the data shown are the means ± SDs from three wells in a representative experiment.