TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| SC19 | Virulent S. suis strain isolated from brain of dead pig | Laboratory collection |
| ΔtroR | troR deletion mutant of strain SC19 | This study |
| CΔtroR | Complemented strain of ΔtroR mutant; Spcr | This study |
| DH5α | Cloning host for recombinant vector | Tsingke |
| Plasmids | ||
| pSET4s | Thermosensitive suicide vector; Spcr | 77 |
| pSET4s-ΔtroR | Knockout vector for troR deletion | This study |
| pSET2 | E. coli-S. suis shuttle vector; Spcr | 79 |
| pSET2-troR | pSET2 containing troR and its promoter | This study |
a
Spcr, spectinomycin resistant.