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. 2021 Sep 28;87(20):e01055-21. doi: 10.1128/AEM.01055-21

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Copyright © 2021 Canon et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Donor strains were selected for their distinct proteolytic profiles. Concentrations of free NH₂ groups after 24 h of fermentation of CDM PROT by donor strains Efa2412, Lla2125, and Lla244 and those present in CDM TRYP and CDM PROT prior to culture. The proteolytic index (PI) obtained for each donor strain cultured in CDM PROT was calculated as the ratio of the peptides and free amino acids (FAA) measurable by OPA during fermentation (OPA_sample) and the OPA_max. The total amount of free NH₂ has been separated into the part related to free amino acids and the one related to peptides, calculated by difference between total and free amino acid-related NH₂ groups, converted in OPA response using the free amino acid dosage. For strain codes, see Table 4.