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. 2021 Sep 24;14:4901–4914. doi: 10.2147/OTT.S286326

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© 2021 Zhang et al.

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miR-425-5p is expressed in the exosomes from BM-MSCs. (A and B) BM-MSCs were treated with control mimic or miR-425-5p mimic. (A) The expression of miR-425-5p was measured by qPCR in the BM-MSCs. (B) The he expression of miR-425-5p was measured by qPCR in the exosomes from BM-MSCs. (C) The morphology of BM-MSCs at passage 3 was observed under a microscope (scale bar: 200 μm). (D) The immunophenotype of BM-MSCs was analyzed using antibodies against CD44, CD90, and CD105 by flow cytometry. (E) The characteristics of exosomes were analyzed by the transmission electron microscopy (TEM) in the BM-MSCs. (F) The expression of CD63, CD81, Tsg101, Grp94, and β-actin was assessed by Western blot analysis in the exosomes from BM-MSCs. Data are presented as mean ± SD. Statistic significant differences were indicated: ***P < 0.001.