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. 2021 Sep 24;14:4901–4914. doi: 10.2147/OTT.S286326

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© 2021 Zhang et al.

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miR-425-5p targets WTAP in AML cells. (A) The interaction of miR-425-5p and WTAP 3ʹ-UTR was identified by bioinformatic analysis using Targetscan (http://www.targetscan.org/vert_72/). (B) The KG-1A and THP-1 cells were treated with miR-425-5p mimic or the control mimic. The luciferase activities of wild type WTAP (WTAP WT) and WTAP with the miR-425-5p-binding site mutant (WTAP MUT) were determined by luciferase reporter gene assays in the cells. (C and D) The exosomes were extracted from the BM-MSCs treated with miR-425-5p mimic or the control mimic, and the KG-1A and THP-1 cells were further treated with the exosomes. (C) The mRNA expression of WTAP was measured by qPCR assays in the cells. (D) The protein expression of WTAP was tested by Western blot analysis in the cells. The results of Western blot analysis were quantified by ImageJ software. (E) The expression levels of WTAP were measured by qPCR assays in the CD34⁺CD38⁻ AML cells from primary AML patients (n = 20) and bone marrow of healthy cases (n = 20). (F) The expression levels of miR-425-5p were assessed by qPCR assays in the KG-1a, NB4, MV411, THP-1 cells, and BM-MSCs. Data are presented as mean ± SD. Statistic significant differences were indicated: **P < 0.01, ***P < 0.001.