Table 2.
Primers and probe sequences for the CTA duplex qRT-PCR assays.
| MAGEA3/ABL duplex (NM_5362.3)a) | |
|---|---|
| CTA primers | TCTTGAGCAGAGGAGTCAGCAC (F); GATCTGGTGACTCGGGAGCA (R) |
| CTA probe | 56-FAM/CTCCCCCAG/ZEN/GGTGACTTCAACTA/3IABkFQ |
| ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
| GATGTAGTTGCTTGGGACCCA (R) | |
| ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
| Amplicon | 176 bp (MAGEA3)+124 bp (ABL) |
| PRAME/ABL duplex (NM_6115.4) | |
| CTA primers | CTGTGCTTGATGGACTTGATGTG (F) |
| GCTGCTCTGCCTCTGTGCTC (R) | |
| CTA probe | 56-FAM/ACCATCTAC/ZEN/TTTTCGCTTCTTTGTCATGGG/3IABkFQ |
| ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
| GATGTAGTTGCTTGGGACCCA (R) | |
| ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
| Amplicon | 216 bp (PRAME)+124 bp (ABL) |
| BAGE2/ABL duplex (NM_1839676.1) | |
| CTA primers | CGGCCAGAGCGGTTTTT (F) |
| CTCCTCCTATTGCTCCTGTTG (R) | |
| CTA probe | 56-FAM/CGTCTCCAT/ZEN/CACCGTGGCTGCCACAA/IABkFG |
| ABL primers | TGGAGATAACACTCTAAGCATAACTAA (F) |
| GATGTAGTTGCTTGGGACCCA (R) | |
| ABL probe | Cy5/CCATTTTTGGTTTGGGCTTCACACCATT/IAbRGSp |
| Amplicon | 150 bp (BAGE2)+124 bp (ABL) |
a)GenBank ref sequence ID for CTA gene.