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. 2021 Mar 29;35(10):2827–2839. doi: 10.1038/s41375-021-01224-2

Fig. 4. Knockdown of MTSS1 enhances araC and DNR resistance of human AML cell lines.

Fig. 4

TF-1 (a), HNT-34 (b), and KG-1 (c) cells were transduced with lentiviral vectors expressing shRNAs targeting MTSS1 (shMTSS1_1, shMTSS1_2) or a non-targeting control shRNA (shCtrl). Downregulation of MTSS1 was validated by immunoblot analysis. Cells were incubated with the indicated concentrations of araC for 2 days or of DNR for 1 day, and stained with AnnexinV. AnnexinV– cells were considered viable, and AnnexinV+ cells apoptotic. Left panels, representative immunoblots; middle panels, quantifications of immunoblots; right panels, AnnexinV assays. Mean + SEM, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. Middle panels: Student’s two-sided t-test, right panels: two-way ANOVA followed by Bonferroni’s post-hoc test.