Skip to main content
. 2021 May 17;35(10):2936–2947. doi: 10.1038/s41375-021-01275-5

Fig. 1. Smad2/3 and Smad4 activation by GDF-11 in human MSCs are inhibited by RAP-536 while neither treatment alters MSC growth or proliferation.

Fig. 1

A Western blot analysis of phospho Smad2/3 (52 kDa) and Smad4 (60 kDa) in healthy and MDS MSCs following 0.1 µg/ml GDF-11/ 10 µg/ml RAP-536 treatment. GAPDH (37 kDa) served as reference protein. B Cell counts of untreated (w/o) or GDF-11/RAP-536-treated healthy and MDS MSCs were determined by trypan blue staining after 2, 4, and 7 days. Values represent mean ± SD in triplicate for N = 3 healthy and N = 5 MDS MSC cell counts normalized to the starting cell number. C Representative light microscopy images showing the morphology of healthy (upper panel) and MDS (lower panel) MSCs at indicated treatment conditions at day 7 (magnification 20×).