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. 2021 May 21;35(10):2948–2963. doi: 10.1038/s41375-021-01267-5

Fig. 4. Co-expression of the host gene Evl and its intronic miR-342 neutralizes their individual hematopoietic differentiation potential.

Fig. 4

a Relative fraction of colony-forming units (CFU-pre B) derived from LSK cells in pre-B cell growth supporting semisolid medium upon Evl, Evl and miR-342, miR-342 or mock transduction. b Relative fraction of colony-forming units (CFU-GM) derived from LSK cells in myeloid and erythroid (My/ery) growth supporting semisolid medium upon miR-342, miR-342 and Evl, Evl or mock transduction. c Predicted miR-342 target genes identified in four different databases (DB: miRWalk, miRanda, RNA22, and TargetScan) and their expression on RNA level (RPKM > 800) in murine primary LSK cell populations [35]. d Pathway analysis using the 1214 in silico-derived miR-342 target genes (Fig. 4c) to describe their molecular functions ranked by the number (no.) of genes in % of total genes within the given category. e One of the top three networks (Score 30–36) targeted by miR-342 identified by in silico analysis (adapted from Ingenuity Pathway Analysis). All identified miR-342 targets within this network are displayed with gray shaded symbols. Solid lines represent direct and dashed lines indirect interactions between molecules based on the IPA database.