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. Author manuscript; available in PMC: 2022 May 5.
Published in final edited form as: J Vasc Res. 2021 May 5;58(5):277–285. doi: 10.1159/000516044

Figure 1. Visualization and quantification of SLC20A1 and RapGEF1.

Figure 1.

Fluorescent immunocytochemistry of cultured HNBSMCs in normal Pi concentration (1.0 mM) probing for A) SLC20A1 (Red) and B) RapGEF1 (Blue) (scale bars = 20μm). C) An overlay was performed showing DAPI (Green) and co-staining between SLC20A1 and RapGEF1 (Purple). D) ImageJ analysis highlights co-localization of staining, with Manders Overlap Coefficients calculated for SLC20A1 on RapGEF1 (S>R) and RapGEF1 on SLC20A1 (R>S) (Co-staining in White).